Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Ethos Biosciences Buffer blocker phosphate casein 1/EA
Dilurex PBS Casein Sucrose Blocking Buffer offers the best price to performance ratio on the market The base system is phosphate buffered saline - perfect for systems that are using PBS as a diluent or reaction buffer Casein is used as the blocking protein which binds to unoccupied hydrophobic or charged sites on assay surfaces preventing unwanted non-specific binding of antibodies enzymes or other proteins leading to clearer more reliable signals Sucrose stabilizes your mixture by preserving the folding integrity of proteins during drying or freezing Choose one of our other blocking buffers if your assay is detecting phosphoproteins uses biotin/streptavidin detection Alkaline Phosphatase detection autoimmune assays or aldehyde coated substrates in case of potential interference
eMolecules tert-Butyl 4-(hydroxymethyl)benzoate | 143726-85-6 | MFCD04973449 | 1g
Ambeed | tert-Butyl 4-(hydroxymethyl)benzoate | 1g | 592282779 | A321260 | | 143726-85-6 | MFCD04973449 | 208.257 | C12H16O3
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Sigma Aldrich Fine Chemicals Biosciences Piperazine BioUltra, anhydrous, >=99.0% (T) | 110-85-0 | MFCD00005953 | 25G
Piperazine BioUltra, anhydrous, >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 25G
Sigma Aldrich Fine Chemicals Biosciences Piperazine | 110-85-0 | MFCD00005953 | 100 g
Piperazine | Purity: ≥99.0% | Mol Wt: 86.14 | 110-85-0 | MFCD00005953 | 100 g
Medchemexpress LLC 2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G
2-Morpholinoethanesulphonic acid monohydrate | 145224-94-8 | 99.9% | 500 G