Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-Buffered Saline (10X, Low Salt), 1000 ml
TRIS-Buffered Saline (10X, Low Salt), 1000 ml.
![Alkali Scientific TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660713.jpg-250.jpg)
Alkali Scientific TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter
TAE 50X Buffer (TRIS Acetate EDTA) is an ultra-concentrated solution used to prepare working buffers for high-resolution nucleic acid electrophoresis. The 1-liter bottle provides a 50X concentration that can be diluted to the required 1X concentration by adding the appropriate amount of water. This buffer is widely used in molecular biology for techniques such as agarose gel electrophoresis, where its low ionic strength minimizes the diffusion of nucleic acid fragments, allowing for better separation. The 50X concentration ensures that laboratories can prepare large quantities of TAE buffer for routine experiments while maintaining buffer performance and stability.
![Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659865.jpg-250.jpg)
Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 1 Kg
BIS TRIS Propane (1,3 Bis(TRIS(hydroxymethyl)methylamino)propane) is a high-purity buffer available in 1 kg powder form. It is commonly used in biochemical and molecular biology applications where a stable pH is essential, particularly in the range of 7.0–9.0. BIS TRIS Propane is effective in protein electrophoresis, enzyme activity assays, and other applications requiring robust buffering in alkaline conditions. The large 1 kg size is ideal for high-throughput laboratories, providing a cost-effective solution for experiments requiring stable pH over extended periods. This buffer is widely used in proteomics, genomics, and pharmaceutical research, where precise pH control is necessary for reproducible results.
![eMolecules 1330764-06-1 | tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | MFCD20230641 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502158274.JPG-250.jpg)
eMolecules 1330764-06-1 | tert-butyl 7-(hydroxymethyl)-5-oxa-2-azaspiro[3.4]octane-2-carboxylate | MFCD20230641 | 1g
Medchem Express | SG3199 | 1mg | 649421525 | HY-101161 | 1595275-71-0 | 584.673 | C33H36N4O6
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Teknova TMN200 Solution, pH 8.0, Research Grade. 2L, Sterile 2 Pack.
200mM Sodium Chloride, 1mM Magnesium Chloride,20mM Tris
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 9.0) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 9 0) - 2x1L (For Research Use and Further Manufacturing Only)
eMolecules 1179360-20-3 | TERT-BUTYL 3-(HYDROXYMETHYL)-1,4-DIAZEPANE-1-CARBOXYLATE HCL | MFCD23702017 | 1g
Ambeed | (R)-Oxiran-2-ylmethyl 4-nitrobenzenesulfonate | 1g | 628567785 | A635502 | 123750-60-7 | MFCD08460169 | 259.230 | C9H9NO6S
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Medchemexpress LLC TAE-1 25mg | 1414469-59-2 | 25 MG
TAE-1 is a small-molecule inhibitor of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) that also inhibits amyloid-β fibril formation and aggregation. It is used in biochemical and cellular research into Alzheimer's disease, cholinesterase function, and amyloid-β aggregation.
- Potent inhibitor of AChE and BuChE.
- Inhibits amyloid-β fibril formation and aggregation.
- Molecular formula C39H51I3N6O9 and molecular weight 1128.57.
- Typical supplied purity about 96.17%.
- Available in small laboratory pack sizes (for example, 25 mg) for research use.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
eMolecules 158144-85-5 | TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | AstaTech | MFCD11044987 | 291.391 | C17H25NO3 | 95.000 | CC(C)(C)OC(=O)N1CCC(CO)(CC1)c1ccccc1 | 1g | 206684235
TERT-BUTYL 4-(HYDROXYMETHYL)-4-PHENYLPIPERIDINE-1-CARBOXYLATE | AstaTech | 158144-85-5 | MFCD11044987 | 291.391 | C17H25NO3 | 95.000 | CC(C)(C)OC(=O)N1CCC(CO)(CC1)c1ccccc1 | 1g | 206684235
![eMolecules 1214727-57-7 | tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000158799.jpg-250.jpg)
eMolecules 1214727-57-7 | tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276
tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | 1214727-57-7 | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276
Kd Medical Inc TBS, 20X, PH7.4, 2000ML
20X Tris Buffer Saline is ready for dilution to a 1X working solution. Used in various research applications. One 2 Liter cubitainer.
Kd Medical Inc TRIS BUFFER, 2M,PH 8.0, BTL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. Tris Solution, 2M, provides a convenient stock solution for preparing Tris buffers> One 500 ml bottle.